Key words
Laboratory exercices in biochemical analysis
Objectives
The care for quality in bio-industries asks for quick, efficient and reliable analysis methods.
Experimental exercices make it possible to learn lab methods with different applications.
These experiments are executed in little groups and the results are discussed.
Topics
Cell culture and transformation techniques.
Analysis of DNA with denaturation profiles, hybridisation, PCR, and agarose gel electrophoresis.
Chromatographic separation of nucleic acids, immunological detection and reactions.
ATP-measurements and the use of luminescence and fluorescence in biochemical analysis.
PAGE and blotting of proteins.
Prerequisites
Profound knowledge and practical experience of enzymology, microbiology and biochemistry.
Final Objectives
General scientific competences
Students are able to perform accurate biochemical analysis and implement new techniques.
General technical competences :
Students are able to understand the theoretical background of practical experiments.
General competences
They should be able to work as a team to reach good results of their biochemical experiments
Specific competences [SC ]
They are able to make a good report and to reflect on results and discussions.
Materials used
::Click here for additional information::
Teacher’s course for practical course.
Study costs
Maximum 10 euro
Study guidance
Possibility to consult the teacher during the experiments.
Electronic: http://docent.hogent.be
Teaching Methods
Laboratory exercises, discussion moments and short presentations .
Assessment
Permanent evaluation for de exercises.
Evaluation of the reports and the presentations.
Lecturer(s)
Katrien Strubbe
An Coorevits
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