Key words
DNA structure, nucleic acid, proteins, qualitatieve & quantitative genetic identification technniques, PCR, quality aspects / IWETO-codes: p320- nucleïnezuren; b220-genetica; b225-plantengenetica; t490-biotechnologie
Objectives
The aim of the course is to give an overview of some of the different available genetic identification techniques. Emphasis will be given not only to the theoretical background but also several practical applications will be discussed.
Competences : The course enables the student to understand and implement the different available genetic identification techniques within the agro sector
Topics
THEORETICAL:
The course gives an lineary organised overview of the different available genetic identification techniques. Initial attention will be given to the basic principles for the amplification and detection of DNA and RNA. Thereafter the genetic identification techniques will be covered in detail, e.g. gelelectroforesis, restriction analysis, chromosome walking, PCR, RFLP, DNA sequencing, protein analysis. Finally an overview of some practical applications within the agro sector will be included.
PRACTICALS:
During the practical excercises different procedures will be covered for obtaining an genetic fingerprint, based on either proteins or DNA, followed by an interpretation of the obtained signals. A number of theoretical excercises will also be included in the excercises. The students will also pay a visit to a biotechnological firm and/or research institution.
Prerequisites
A basic knowledge of genetics, biochemistry and biotechnology.
Final Objectives
Thorough knowledge and implementation of the research and analytical instrumentarium of the genetic analyses
Materials used
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Syllabus
Textbooks and scientific papers; websites
Study costs
Cost syllabus and practicum notes: 8,0 Euro
Study guidance
Additional information can always be asked.
Teaching Methods
Lectures; laboratory excercises and visit to research institution
Assessment
Theory: Written examination
Practicals: permanent evaluation and quotation of the reports
Lecturer(s)
T.: Kathy Messens
P: Kathy Messens, Nicolas Gryson
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