Key words
Objectives
The students get knownledge on analytical technics, based on immunological, enzymatical; modern extraction methods and specialized detection methods.
They get the ability to preform, understand and evaluate laboratory protocols on isolation, purification and characterisation of biological substances, such as proteins and nucleic acids.
Topics
Theory :
- isolation technics of nucleic acids and of proteins
- electroforesis of proteins
- electroforesis of nucleic acids
- hybridisation and blotting technics
- fluorescence based technics
- cultivation of animal and plant cells
- labeling of nucleic acids and proteins
- antisense method
- ATP detection and enzymatic analysis of food and food components
- biosafety in a biotech lab
Practical :
- Immunodiffusion and immuno-electoroforese and ELISA
- enzymatic foodanalysis with a kit
- restriction enzymanalysis
- agarose electroforesis and Southern blotting of DNA
- PAGE and western blotting of proteins
- quantitative ATP detection with fluorescence spectroscopy
Prerequisites
Final Objectives
Materials used
- teacher's course
- scientific & technical literature
Study costs
7.5 euro and facultative a recent scientific cell biology book of 70-75 euro
Study guidance
Teaching Methods
- lectures
- demonstrations
- exercises
Assessment
- Scoring system :
Theory:
oral examination
Practical:
permanent evaluation
Lecturer(s)
Katrien Strubbe
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