Key words
Methods of biochemical analysis
Objectives
Quality control needs accurate methods for rapid analysis. The identification and the quantification of specific biomolecules are possible by use of a great diversity of biochemical methods for analyzing even complex samples.
Prerequisites
Basic knowledge of biochemistry, enzymology and microbiology.
Topics
- The use of agarose and p.a.a. gels in diverse electrophoretic separation methods for proteins and nucleic acids.
- Detection of biomolecules by fluorescent dyes.
- Immunological methods: diffusion, electrophoresis, ELISA
- The detection of microbiological contamination by looking for ATP, lipides, enzymes and toxines
- DNA-technology: in situ hybridisation, blotting, PCR and deduced methods.
- Comparision of the classical microbiological methods and the new DBA-based methods
Teaching Methods
lectures - demonstrations
Materials used
teacher's course - other textbook(s) - school library materials
Study guidance
Assessment
Theoretical course: oral examination
Practical course: evaluation during course - report of the results.
Study costs
Lecturer(s)
Raphaël ROGIERS
Language
Dutch
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